Journal: Neuron

Article Title: Spatial clustering of inhibition in mouse primary visual cortex

doi: 10.1016/j.neuron.2019.09.020

Figure Lengend Snippet: Highlights

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Donkey anti mouse IgG Cy5 Jackson Immunoresearch Cat# 715-175-151 Donkey anti goat IgG biotin-SP conjugate Sigma-Aldrich Cat# AP180B Goat anti choline acetyl transferase Sigma-Aldrich Cat# AB144P Goat anti rat IgG Alexa Fluor 647 ThermoFisher Cat# A21247 Mouse monoclonal anti Ankyrin G antibody NeuroMab Cat# N106/36 Rat monoclonal anti M2 muscarinic acetylcholine receptor antibody Sigma-Aldrich Cat# MAB367 Labeled reagents Alexa 594 hydrazide ThermoFisher Cat# A10438 NeutrAvidin Oregon Green 488 ThermoFisher Cat# A6374 Neutralite Avidin-CY5 SouthernBiotech Cat# 7200–15 Streptavidin, Alexa Fluor 647 conjugate ThermoFisher Cat # {"type":"entrez-protein","attrs":{"text":"S21374","term_id":"99986","term_text":"pir||S21374"}} S21374 Biochemical Reagents 4-Aminopyridine Tocris Bioscience Cat# 0940 DNQX (6,7-dinitroquinoxaline-2,3-dione) Tocris Biochemical Cat# 2312 Picrotoxin Tocris Bioscience Cat# 1128 (RS) CPP ((RS)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid Tocris Bioscience Cat# 0173 Tetrodotoxin Tocris Bioscience Cat# 1078 Experimental Models: Organisms/Strains Ai9 (Gt[ROSA]26Sor tm9(CAG-tdTomato)Hze ) Jackson Laboratory Stock No: 007909 C57BL/6J Jackson Laboratory Stock No: 000664 Chrm2-tdT-D knock-in mice (Bg6.Cg-Chrm 2tm1.1Hze /J) Jackson Laboratory Stock No: 030330 Chrm2 M2R−/− (B6N.129S4(Cg)-Chrm2 tmJwe /J) Jackson Laboratory Stock No: 030162 PV-Cre (Bg.

Techniques: Labeling, Avidin-Biotin Assay, Knock-In, Virus, Plasmid Preparation, Software

Journal: Frontiers in Neuroanatomy

Article Title: Neurogliaform cells mediate feedback inhibition in the medial entorhinal cortex

doi: 10.3389/fnana.2022.779390

Figure Lengend Snippet: Layer I neurogliaform cells receive excitatory monosynaptic inputs from layer II pyramidal cells. (A) Confocal image of a biocytin-filled layer I neurogliaform cell surrounded by ChR2-mCherry-positive axons (yellow) (left). The recorded cell shows GABAARα1 (middle) and Reelin (right) immunopositivity (scale bars: 15 μm). (B) Representative reconstruction of somatic locations in a 40 μm thick section of the mouse medial entorhinal cortex showing layer I GABAARα1 (red circle) positive cells together with GABAARα1 and Reelin positive cells (green triangle) (scale bar: 500 μm). (C) The postsynaptic effects seen on neurogliaform cells (black, firing pattern in inset) were reduced by 1 μM TTX (green). Of note, 1 mM 4-AP not only recovered but also increased the amplitude of the postsynaptic effect, indicating monosynaptic input [RMP: –65 mV, excitatory postsynaptic potential (EPSP) amplitude: before TTX application 3.1 ± 0.6 mV, after TTX application 0.3 ± 0.2 mV and after 4-AP application 9.8 ± 2.9 mV, n = 4]. (D) The voltage response of the neurogliaform cell to a 5 ms photo-stimulation (black, single sweep EPSP, n = 34) and the disappearance of the effect after 10 μM CNQX + 10 μM NBQX wash in (red) (RMP: –65 mV, scale bars: 3 mV and 20 ms). Inset: response of the recorded cell to 1 s current injection (RMP: –65 mV and –100 and +150 pA). Note that the blue bars on panel (C) and (D) represent the 5 ms long light-pulses.

Article Snippet: In some experiments, 10 μM NBQX (NBQX disodium salt hydrate; CAS 118876-58-7; N183, Sigma-Aldrich), 10 μM CNQX (Cat. No. 0190, Tocris), 1 μM TTX (Tetrodotoxin, Cat. No. 1078, Tocris), and 4-aminopyridine (CAS 504-24-5; 275875, Sigma-Aldrich) were used.

Techniques: Injection